BAIT

RGA1

F2I9.19, F2I9_19, REPRESSOR OF GA, REPRESSOR OF GA1-3 1, RGA, AT2G01570
DELLA protein RGA
Arabidopsis thaliana (Columbia)
PREY

AT3G45260

C2H2-like zinc finger protein
GO Process (1)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

DELLA protein functions as a transcriptional activator through the DNA binding of the INDETERMINATE DOMAIN family proteins.

Yoshida H, Hirano K, Sato T, Mitsuda N, Nomoto M, Maeo K, Koketsu E, Mitani R, Kawamura M, Ishiguro S, Tada Y, Ohme-Takagi M, Matsuoka M, Ueguchi-Tanaka M

DELLA protein is a key negative regulator of gibberellin (GA) signaling. Although how DELLA regulates downstream gene expression remains unclear, DELLA has been proposed to function as a transcriptional activator. However, because DELLA lacks a DNA-binding domain, intermediate protein(s) mediating the DELLA/DNA interaction are believed to be necessary for activating DELLA target genes. Here, using yeast hybrid screenings, we identified ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 12, 2014; 0(0); [Pubmed: 24821766]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RGA1 AT3G45260
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
1173375

Curated By

  • BioGRID