An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

DELLA protein functions as a transcriptional activator through the DNA binding of the INDETERMINATE DOMAIN family proteins.

Yoshida H, Hirano K, Sato T, Mitsuda N, Nomoto M, Maeo K, Koketsu E, Mitani R, Kawamura M, Ishiguro S, Tada Y, Ohme-Takagi M, Matsuoka M, Ueguchi-Tanaka M

DELLA protein is a key negative regulator of gibberellin (GA) signaling. Although how DELLA regulates downstream gene expression remains unclear, DELLA has been proposed to function as a transcriptional activator. However, because DELLA lacks a DNA-binding domain, intermediate protein(s) mediating the DELLA/DNA interaction are believed to be necessary for activating DELLA target genes. Here, using yeast hybrid screenings, we identified ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 12, 2014; 0(0); [Pubmed: 24821766]

Throughput

Low Throughput

Additional Notes

BiFC assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RGA1 IDD5	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yoshida H (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RGA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA, ISS, TAS]transcription regulatory region DNA binding [IPI]

Gene Ontology Cellular Component

IDD5

Gene Ontology Biological Process

Gene Ontology Molecular Function

nucleic acid binding [ISS]sequence-specific DNA binding transcription factor activity [ISS]zinc ion binding [ISS]