MGP
Gene Ontology Biological Process
Gene Ontology Molecular Function
RGA1
Gene Ontology Biological Process
- gibberellic acid mediated signaling pathway [TAS]
- hyperosmotic salinity response [IGI]
- jasmonic acid mediated signaling pathway [IGI]
- negative regulation of gibberellic acid mediated signaling pathway [IGI]
- negative regulation of seed germination [IGI]
- regulation of protein catabolic process [IMP]
- regulation of reactive oxygen species metabolic process [IGI]
- regulation of seed dormancy process [IGI]
- regulation of seed germination [IGI]
- regulation of transcription, DNA-templated [TAS]
- response to abscisic acid [IGI]
- response to ethylene [IGI]
- response to far red light [IEP]
- response to salt stress [IGI]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
DELLA protein functions as a transcriptional activator through the DNA binding of the INDETERMINATE DOMAIN family proteins.
DELLA protein is a key negative regulator of gibberellin (GA) signaling. Although how DELLA regulates downstream gene expression remains unclear, DELLA has been proposed to function as a transcriptional activator. However, because DELLA lacks a DNA-binding domain, intermediate protein(s) mediating the DELLA/DNA interaction are believed to be necessary for activating DELLA target genes. Here, using yeast hybrid screenings, we identified ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RGA1 MGP | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | 1173372 | |
| RGA1 MGP | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| RGA1 MGP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID