BAIT

NUP100

NSP100, FG-nucleoporin NUP100, [NUP100+], L000001292, YKL068W
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

GSP1

CNR1, CST17, Ran GTPase GSP1, L000000736, YLR293C
Ran GTPase; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; GSP1 has a paralog, GSP2, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Altering nuclear pore complex function impacts longevity and mitochondrial function in S. cerevisiae.

Lord CL, Timney BL, Rout MP, Wente SR

The eukaryotic nuclear permeability barrier and selective nucleocytoplasmic transport are maintained by nuclear pore complexes (NPCs), large structures composed of ∼ 30 proteins (nucleoporins [Nups]). NPC structure and function are disrupted in aged nondividing metazoan cells, although it is unclear whether these changes are a cause or consequence of aging. Using the replicative life span (RLS) of Saccharomyces cerevisiae as ... [more]

J. Cell Biol. Mar. 16, 2015; 208(6);729-44 [Pubmed: 25778920]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: replicative lifespan (APO:0000317)

Additional Notes

  • Inhibition of GSP1 function using the gsp1-322 mutant largely suppressed the extended RLSs of nup100 mutant cells

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NUP100 GSP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1292BioGRID
2053752

Curated By

  • BioGRID