A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

InsP6-sensitive variants of the Gle1 mRNA export factor rescue growth and fertility defects of the ipk1 low-phytic-acid mutation in Arabidopsis.

Lee HS, Lee DH, Cho HK, Kim SH, Auh JH, Pai HS

Myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP(6)), also known as phytic acid, accumulates in large quantities in plant seeds, serving as a phosphorus reservoir, but is an animal antinutrient and an important source of water pollution. Here, we report that Gle1 (GLFG lethal 1) in conjunction with InsP(6) functions as an activator of the ATPase/RNA helicase LOS4 (low expression of osmotically responsive genes 4), which ... [more]

Plant Cell Feb. 01, 2015; 27(2);417-31 [Pubmed: 25670768]

Throughput

Low Throughput

Additional Notes

BiFC

Curated By

BioGRID

Interaction Summary

LOS4

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent helicase activity [ISS]RNA helicase activity [ISS]RNA-dependent ATPase activity [IDA]

Gene Ontology Cellular Component

IPK1