SMURF2
Gene Ontology Biological Process
- BMP signaling pathway [TAS]
- gene expression [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [NAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA]
- regulation of transforming growth factor beta receptor signaling pathway [NAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IBA, TAS]
- ubiquitin-dependent SMAD protein catabolic process [IDA]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
RAB13
Gene Ontology Biological Process
- GTP catabolic process [ISO]
- Rab protein signal transduction [IBA]
- cellular response to insulin stimulus [ISO]
- cortical actin cytoskeleton organization [IBA, ISO]
- endocytic recycling [IMP, ISO]
- endosomal transport [ISO]
- endothelial cell chemotaxis [IMP]
- establishment of Sertoli cell barrier [ISO]
- establishment of protein localization to plasma membrane [ISO]
- intracellular protein transport [TAS]
- neuron projection development [ISO]
- protein kinase A signaling [ISO]
- protein localization to cell leading edge [IMP]
- protein localization to plasma membrane [ISO]
- tight junction assembly [IBA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cell [IMP]
- cell-cell junction [IDA]
- cytoplasm [ISO]
- cytoplasmic membrane-bounded vesicle [TAS]
- cytoplasmic vesicle [ISO]
- endocytic vesicle [IDA, ISO]
- endosome [IBA]
- extracellular vesicular exosome [ISO]
- extrinsic component of plasma membrane [TAS]
- insulin-responsive compartment [ISO]
- intracellular [IMP]
- lamellipodium [IDA]
- lateral plasma membrane [ISO]
- neuron projection [ISO]
- plasma membrane [ISO]
- recycling endosome [ISO]
- tight junction [IDA, ISO]
- trans-Golgi network [ISO]
Affinity Capture-Luminescence
An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.
Publication
High-throughput mapping of a dynamic signaling network in mammalian cells.
Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor-beta (TGFbeta) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFbeta pathway ... [more]
Throughput
- High Throughput
Curated By
- BioGRID