An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

BioID-based Identification of Skp Cullin F-box (SCF)Î²-TrCP1/2 E3 Ligase Substrates.

Coyaud E, Mis M, Laurent EM, Dunham WH, Couzens AL, Robitaille M, Gingras AC, Angers S, Raught B

The identification of ubiquitin E3 ligase substrates has been challenging, due in part to low-affinity, transient interactions, the rapid degradation of targets and the inability to identify proteins from poorly soluble cellular compartments. SCF(Î²-TrCP1) and SCF(Î²-TrCP2) are well-studied ubiquitin E3 ligases that target substrates for proteasomal degradation, and play important roles in Wnt, Hippo, and NFÎºB signaling. Combining 26S proteasome ... [more]

Mol. Cell Proteomics Jul. 01, 2015; 14(7);1781-95 [Pubmed: 25900982]

Throughput

High Throughput

Additional Notes

in the presence of MG132

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FBXW11 HNRNPDL	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Low TY (2014)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FBXW11

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin-protein transferase activity [IDA, NAS]

Gene Ontology Cellular Component

HNRNPDL

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded DNA binding [IDA]poly(A) RNA binding [IDA]poly(A) binding [IDA]poly(G) binding [IDA]single-stranded DNA binding [IDA]