BAIT

NOB1

YOR29-07, YOR056C
Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

BUD23

YCR047C
Methyltransferase; methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The rRNA methyltransferase Bud23 shows functional interaction with components of the SSU processome and RNase MRP.

Sardana R, White JP, Johnson AW

Bud23 is responsible for the conserved methylation of G1575 of 18S rRNA, in the P-site of the small subunit of the ribosome. bud23Δ mutants have severely reduced small subunit levels and show a general failure in cleavage at site A2 during rRNA processing. Site A2 is the primary cleavage site for separating the precursors of 18S and 25S rRNAs. Here, ... [more]

RNA Jun. 01, 2013; 19(6);828-40 [Pubmed: 23604635]

Throughput

  • Low Throughput

Additional Notes

  • Figure 1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BUD23 NOB1
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

High-BioGRID
1174359

Curated By

  • BioGRID