BAIT

BUD23

YCR047C
Methyltransferase; methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
Saccharomyces cerevisiae (S288c)
PREY

DHR2

YKL078W
Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

The rRNA methyltransferase Bud23 shows functional interaction with components of the SSU processome and RNase MRP.

Sardana R, White JP, Johnson AW

Bud23 is responsible for the conserved methylation of G1575 of 18S rRNA, in the P-site of the small subunit of the ribosome. bud23Δ mutants have severely reduced small subunit levels and show a general failure in cleavage at site A2 during rRNA processing. Site A2 is the primary cleavage site for separating the precursors of 18S and 25S rRNAs. Here, ... [more]

RNA Jun. 01, 2013; 19(6);828-40 [Pubmed: 23604635]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • Table 1

Curated By

  • BioGRID