Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The molecular mechanism of sporocyteless/nozzle in controlling Arabidopsis ovule development.

Wei B, Zhang J, Pang C, Yu H, Guo D, Jiang H, Ding M, Chen Z, Tao Q, Gu H, Qu LJ, Qin G

Ovules are essential for plant reproduction and develop into seeds after fertilization. Sporocyteless/nozzle (SPL/NZZ) has been known for more than 15 years as an essential factor for ovule development in Arabidopsis, but the biochemical nature of SPL function has remained unsolved. Here, we demonstrate that SPL functions as an adaptor-like transcriptional repressor. We show that SPL recruits topless/topless-related (TPL/TPR) co-repressors ... [more]

Cell Res. Jan. 01, 2015; 25(1);121-34 [Pubmed: 25378179]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SPL TCP17	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Wei B (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SPL

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sequence-specific DNA binding transcription factor activity [IMP, ISS]

Gene Ontology Cellular Component

TCP17