RDR6
Gene Ontology Biological Process
- RNA interference [IMP]
- carpel development [IGI]
- defense response to virus [IMP]
- gene silencing by RNA [IMP]
- gynoecium development [IMP]
- leaf development [IGI]
- posttranscriptional gene silencing [TAS]
- production of miRNAs involved in gene silencing by miRNA [IMP]
- production of siRNA involved in RNA interference [TAS]
- production of ta-siRNAs involved in RNA interference [IMP]
- recognition of pollen [IMP]
- virus induced gene silencing [IMP]
Gene Ontology Molecular Function
SGS3
Gene Ontology Biological Process
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
In Planta Recognition of a Double-Stranded RNA Synthesis Protein Complex by a Potexviral RNA Silencing Suppressor.
RNA silencing plays an important antiviral role in plants and invertebrates. To counteract antiviral RNA silencing, most plant viruses have evolved viral suppressors of RNA silencing (VSRs). TRIPLE GENE BLOCK PROTEIN1 (TGBp1) of potexviruses is a well-characterized VSR, but the detailed mechanism by which it suppresses RNA silencing remains unclear. We demonstrate that transgenic expression of TGBp1 of plantago asiatica ... [more]
Throughput
- Low Throughput
Additional Notes
- proteins expressed in Nicotiana benthamiana leaves
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SGS3 RDR6 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | - |
Curated By
- BioGRID