ROM2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RHO1
Gene Ontology Biological Process
- actin cytoskeleton organization [IMP]
- actin cytoskeleton reorganization [IGI, IPI]
- budding cell bud growth [IGI, IMP]
- positive regulation of endocytosis [IMP]
- positive regulation of protein kinase C signaling [IDA, IGI, IMP, IPI]
- regulation of cell size [IMP]
- regulation of cell wall (1->3)-beta-D-glucan biosynthetic process [IDA, IMP]
- regulation of exocyst localization [IMP]
- regulation of fungal-type cell wall organization [IMP]
- regulation of protein localization [IMP]
- regulation of vacuole fusion, non-autophagic [IMP]
- small GTPase mediated signal transduction [IDA, IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis.
In yeast and animal cytokinesis, the small guanosine triphosphatase (GTPase) Rho1/RhoA has an established role in formation of the contractile actomyosin ring, but its role, if any, during cleavage-furrow ingression and abscission is poorly understood. Through genetic screens in yeast, we found that either activation of Rho1 or inactivation of another small GTPase, Cdc42, promoted secondary septum (SS) formation, which ... [more]
Throughput
- Low Throughput
Additional Notes
- BiFC
- Figure 8
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ROM2 RHO1 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 155171 | |
RHO1 ROM2 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 155172 | |
ROM2 RHO1 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 163316 | |
RHO1 ROM2 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 255428 | |
ROM2 RHO1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 519528 | |
ROM2 RHO1 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 519522 | |
RHO1 ROM2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
RHO1 ROM2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID