BAIT

HIST1H2AA

H2AA, H2AFR, bA317E16.2
histone cluster 1, H2aa
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens
PREY

HDAC1

GON-10, HD1, RPD3, RPD3L1, RP4-811H24.2
histone deacetylase 1
GO Process (33)
GO Function (18)
GO Component (10)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Histone deacetylase 1 and p300 can directly associate with chromatin and compete for binding in a mutually exclusive manner.

Li X, Yang H, Huang S, Qiu Y

Lysine acetyltransferases (KATs) and histone deacetylases (HDACs) are important epigenetic modifiers and dynamically cycled on active gene promoters to regulate transcription. Although HDACs are recruited to gene promoters and DNA hypersensitive sites through interactions with DNA binding factors, HDAC activities are also found globally in intergenic regions where DNA binding factors are not present. It is suggested that HDACs are ... [more]

PLoS ONE Apr. 12, 2014; 9(4);e94523 [Pubmed: 24722339]

Throughput

  • Low Throughput

Curated By

  • BioGRID