RAGC-D
Gene Ontology Biological Process
Gene Ontology Molecular Function
GIG
Gene Ontology Biological Process
- TORC1 signaling [IMP]
- cellular response to DNA damage stimulus [IMP]
- cellular response to hypoxia [IMP]
- determination of adult lifespan [IMP]
- insulin-like growth factor receptor signaling pathway [IBA]
- larval midgut histolysis [IGI]
- lateral inhibition [IMP]
- lipid metabolic process [IGI]
- negative regulation of TOR signaling [IBA]
- negative regulation of Wnt signaling pathway [IBA]
- negative regulation of cell growth [IGI, IMP]
- negative regulation of cell proliferation [IGI, IMP]
- negative regulation of cell size [IGI, IMP]
- negative regulation of insulin receptor signaling pathway [IGI]
- negative regulation of organ growth [IGI]
- negative regulation of synaptic growth at neuromuscular junction [IMP]
- oogenesis [IMP]
- phagocytosis [IMP]
- positive regulation of GTPase activity [IBA]
- positive regulation of Ras GTPase activity [IDA]
- positive regulation of autophagy [IMP]
- positive regulation of protein phosphorylation [IDA]
- protein kinase B signaling [IBA]
- regulation of cell cycle [IMP]
- regulation of phosphorylation [IDA]
- response to nutrient [IMP]
- signal transduction [IGI]
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Regulation of TORC1 in response to amino acid starvation via lysosomal recruitment of TSC2.
TOR complex 1 (TORC1) is a potent anabolic regulator of cellular growth and metabolism. When cells have sufficient amino acids, TORC1 is active due to its lysosomal localization mediated via the Rag GTPases. Upon amino acid removal, the Rag GTPases release TORC1, causing it to become cytoplasmic and inactive. We show here that, upon amino acid removal, the Rag GTPases ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAGC-D GIG | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID