BAIT
NKX2-5
Csx, Nkx-2.5, Nkx2.5, tinman
NK2 homeobox 5
GO Process (66)
GO Function (16)
GO Component (4)
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- Purkinje myocyte differentiation [IMP]
- adult heart development [IMP, ISO]
- apoptotic process involved in heart morphogenesis [IMP]
- atrial cardiac muscle cell development [TAS]
- atrial septum morphogenesis [ISO]
- atrioventricular node cell development [IMP]
- atrioventricular node cell fate commitment [IMP]
- atrioventricular node development [IMP]
- bundle of His development [IGI]
- canonical Wnt signaling pathway [IDA]
- cardiac conduction system development [IGI, ISO]
- cardiac muscle cell differentiation [IDA, IGI, IMP, TAS]
- cardiac muscle cell proliferation [IGI]
- cardiac muscle contraction [IMP]
- cardiac muscle tissue development [IMP]
- cardiac muscle tissue morphogenesis [ISO]
- cardiac ventricle formation [IGI]
- cardiac ventricle morphogenesis [IMP]
- cardiovascular system development [IGI]
- cell differentiation [IGI]
- embryonic heart tube development [IGI]
- embryonic heart tube left/right pattern formation [IMP]
- endoderm development [TAS]
- heart contraction [IGI]
- heart development [IGI, IMP]
- heart looping [IMP]
- heart morphogenesis [IMP]
- heart trabecula formation [IMP]
- hemopoiesis [IMP]
- negative regulation of apoptotic process [IGI]
- negative regulation of canonical Wnt signaling pathway [IDA]
- negative regulation of cardiac muscle cell apoptotic process [IMP, ISO]
- negative regulation of myotube differentiation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- outflow tract morphogenesis [IGI, IMP]
- outflow tract septum morphogenesis [ISO]
- pharyngeal system development [IGI]
- positive regulation of cardioblast differentiation [IMP]
- positive regulation of cell proliferation [IGI]
- positive regulation of gene expression [IDA]
- positive regulation of heart contraction [IMP]
- positive regulation of neuron differentiation [ISO]
- positive regulation of sodium ion transport [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription initiation from RNA polymerase II promoter [IDA]
- positive regulation of transcription via serum response element binding [IDA]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation of voltage-gated calcium channel activity [IMP]
- proepicardium development [IMP]
- pulmonary myocardium development [IMP]
- regulation of cardiac muscle cell proliferation [IDA]
- regulation of cardiac muscle contraction [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- right ventricular cardiac muscle tissue morphogenesis [ISO]
- sarcomere organization [IGI]
- septum secundum development [ISO]
- spleen development [IGI, ISO, TAS]
- thyroid gland development [IMP, ISO]
- tongue development [TAS]
- transcription from RNA polymerase II promoter [IMP]
- vasculogenesis [IMP]
- ventricular cardiac muscle cell development [TAS]
- ventricular cardiac myofibril assembly [IMP]
- ventricular septum morphogenesis [ISO]
- ventricular trabecula myocardium morphogenesis [IMP]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription cofactor activity [IMP]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IMP]
- chromatin binding [IDA, ISO]
- histone deacetylase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- serum response element binding [IDA]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription cofactor activity [IMP]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IMP]
- chromatin binding [IDA, ISO]
- histone deacetylase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- serum response element binding [IDA]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
UBC
2700054O04Rik, AI194771, Rps27a, TI-225, Uba52, Ubb
ubiquitin C
GO Process (1)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Fbxo25 controls Tbx5 and Nkx2-5 transcriptional activity to regulate cardiomyocyte development.
The ubiquitin-proteasome system (UPS) plays an important role in protein quality control, cellular signalings, and cell differentiation through the regulated turnover of key transcription factors in cardiac tissue. However, the molecular mechanism underlying Fbxo25-mediated ubiquitination of cardiac transcription factors remains elusive. We report that an Fbxo25-mediated SCF ubiquitination pathway regulates the protein levels and activities of Tbx5 and Nkx2-5 based ... [more]
Biochim. Biophys. Acta Jun. 01, 2015; 1849(6);709-21 [Pubmed: 25725482]
Throughput
- Low Throughput
Curated By
- BioGRID