AASDHPPT
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
STIL
Gene Ontology Biological Process
- cell proliferation [TAS]
- centrosome duplication [IDA, IMP]
- determination of left/right symmetry [ISS]
- embryonic axis specification [ISS]
- floor plate development [ISS]
- forebrain development [ISS]
- heart looping [ISS]
- in utero embryonic development [ISS]
- mitotic spindle organization [IMP]
- multicellular organism growth [ISS]
- negative regulation of apoptotic process [ISS]
- neural tube closure [ISS]
- neural tube development [ISS]
- notochord development [ISS]
- protein localization to centrosome [IMP]
- smoothened signaling pathway [ISS]
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Quantitative Score
- 0.845939962 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.845939962, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
STIL AASDHPPT | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 1434244 |
Curated By
- BioGRID