BAIT
SPRY2
hSPRY2
sprouty homolog 2 (Drosophila)
GO Process (10)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of peptidyl-threonine phosphorylation [IDA]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of protein serine/threonine kinase activity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
VANGL2
LPP1, LTAP, STB1, STBM, STBM1
VANGL planar cell polarity protein 2
GO Process (6)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Cell Jul. 16, 2015; 162(2);425-40 [Pubmed: 26186194]
Quantitative Score
- 0.984912833 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.984912833, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Curated By
- BioGRID