AAGAB
Gene Ontology Cellular Component
AP1G1
Gene Ontology Biological Process
- Golgi to lysosome transport [IMP]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- endosome to melanosome transport [IMP]
- melanosome organization [IC]
- membrane organization [TAS]
- positive regulation of natural killer cell degranulation [IMP]
- positive regulation of natural killer cell mediated cytotoxicity [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- regulation of defense response to virus by virus [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- AP-type membrane coat adaptor complex [TAS]
- Golgi apparatus [IDA]
- Golgi membrane [TAS]
- clathrin-coated vesicle [IDA]
- clathrin-coated vesicle membrane [ISS]
- cytoplasm [IDA]
- cytoplasmic vesicle membrane [TAS]
- cytosol [TAS]
- intracellular membrane-bounded organelle [IDA]
- lysosomal membrane [TAS]
- membrane [IDA]
- recycling endosome [IDA]
- trans-Golgi network membrane [TAS]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Quantitative Score
- 0.999910318 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.999910318, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AAGAB AP1G1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9991 | BioGRID | 2231841 | |
| AAGAB AP1G1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9991 | BioGRID | 3031466 | |
| AAGAB AP1G1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2708326 |
Curated By
- BioGRID