ASB4
Gene Ontology Biological Process
Gene Ontology Cellular Component
HIF1AN
Gene Ontology Biological Process
- cellular response to hypoxia [TAS]
- negative regulation of Notch signaling pathway [IDA]
- negative regulation of transcription from RNA polymerase II promoter in response to hypoxia [IDA]
- oxidation-reduction process [IDA]
- peptidyl-asparagine hydroxylation [IDA, IMP]
- peptidyl-aspartic acid hydroxylation [IDA]
- peptidyl-histidine hydroxylation [IDA]
- positive regulation of myoblast differentiation [IDA]
- positive regulation of vasculogenesis [NAS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
Gene Ontology Molecular Function- NF-kappaB binding [IPI]
- Notch binding [IPI]
- ankyrin repeat binding [IPI]
- carboxylic acid binding [IDA]
- cofactor binding [IDA]
- iron ion binding [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [EXP]
- oxygen sensor activity [NAS]
- peptidyl-asparagine 3-dioxygenase activity [IDA, IMP]
- peptidyl-histidine dioxygenase activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- zinc ion binding [IDA]
- NF-kappaB binding [IPI]
- Notch binding [IPI]
- ankyrin repeat binding [IPI]
- carboxylic acid binding [IDA]
- cofactor binding [IDA]
- iron ion binding [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [EXP]
- oxygen sensor activity [NAS]
- peptidyl-asparagine 3-dioxygenase activity [IDA, IMP]
- peptidyl-histidine dioxygenase activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Quantitative Score
- 0.99724328 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.99724328, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ASB4 HIF1AN | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9323 | BioGRID | 2254728 | |
| ASB4 HIF1AN | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9802 | BioGRID | 3065564 | |
| ASB4 HIF1AN | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ASB4 HIF1AN | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID