BAIT
STK19
D6S60, D6S60E, G11, HLA-RP1, RP1, DADB-112B14.2
serine/threonine kinase 19
GO Process (1)
GO Function (0)
GO Component (0)
Gene Ontology Biological Process
Homo sapiens
PREY
SLC29A1
ENT1
solute carrier family 29 (equilibrative nucleoside transporter), member 1
GO Process (4)
GO Function (0)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.
High-throughput (HTP) protein-interaction assays, such as the yeast two-hybrid (Y2H) system, are enormously useful in predicting the functions of novel gene-products. HTP-Y2H screens typically do not include all of the reconfirmation and specificity tests used in small-scale studies, but the effects of omitting these steps have not been assessed. We performed HTP-Y2H screens that included all standard controls, using the ... [more]
Genomics Jan. 01, 2004; 83(1);153-67 [Pubmed: 14667819]
Throughput
- Low Throughput
Curated By
- BioGRID