TF
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical plasma membrane [IDA]
- basal part of cell [IDA]
- basal plasma membrane [IDA]
- blood microparticle [IDA]
- cell surface [IDA]
- coated pit [IDA]
- cytoplasmic membrane-bounded vesicle [IDA]
- early endosome [IDA]
- endocytic vesicle [IDA]
- endosome membrane [TAS]
- extracellular region [NAS, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- late endosome [IDA]
- perinuclear region of cytoplasm [IDA]
- recycling endosome [IDA]
- secretory granule lumen [TAS]
- vesicle [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Quantitative Score
- 0.979991845 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.979991845, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SYT17 TF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9532 | BioGRID | 2251247 | |
| SYT17 TF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9849 | BioGRID | 3065371 |
Curated By
- BioGRID