BAIT
PIP5K1A
RP11-68I18.9
phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
GO Process (16)
GO Function (3)
GO Component (10)
Gene Ontology Biological Process
- actin cytoskeleton reorganization [IMP]
- activation of Rac GTPase activity [IMP]
- cell chemotaxis [IMP]
- cell migration [NAS]
- focal adhesion assembly [IMP]
- glycerophospholipid metabolic process [TAS]
- keratinocyte differentiation [TAS]
- phagocytosis [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol phosphorylation [IDA, TAS]
- phospholipid biosynthetic process [IDA]
- phospholipid metabolic process [TAS]
- protein targeting to plasma membrane [IMP]
- ruffle assembly [IMP]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
FLNC
ABP-280, ABP280A, ABPA, ABPL, FLN2, MFM5, MPD4
filamin C, gamma
GO Process (1)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Cell Jul. 16, 2015; 162(2);425-40 [Pubmed: 26186194]
Quantitative Score
- 0.809463412 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.809463412, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Curated By
- BioGRID