BAIT
ADCYAP1
PACAP
adenylate cyclase activating polypeptide 1 (pituitary)
GO Process (15)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
- activation of adenylate cyclase activity [TAS]
- cAMP-mediated signaling [IMP]
- cell-cell signaling [TAS]
- female pregnancy [TAS]
- neuron projection development [IDA]
- neuropeptide signaling pathway [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of Rap GTPase activity [IDA]
- positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway [IDA]
- positive regulation of protein kinase activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of G-protein coupled receptor protein signaling pathway [IDA]
- regulation of protein localization [IDA]
- transmembrane receptor protein tyrosine kinase signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
MKLN1
TWA2
muskelin 1, intracellular mediator containing kelch motifs
GO Process (1)
GO Function (0)
GO Component (0)
Gene Ontology Biological Process
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Cell Jul. 16, 2015; 162(2);425-40 [Pubmed: 26186194]
Quantitative Score
- 0.814407641 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.814407641, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Curated By
- BioGRID