C1ORF186
PKN1
Gene Ontology Biological Process
Gene Ontology Molecular Function- GTP-Rho binding [IDA]
- Rac GTPase binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- histone binding [IDA]
- histone deacetylase binding [IDA]
- histone kinase activity (H3-T11 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA, IMP]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA]
- GTP-Rho binding [IDA]
- Rac GTPase binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- histone binding [IDA]
- histone deacetylase binding [IDA]
- histone kinase activity (H3-T11 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA, IMP]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Quantitative Score
- 0.999964633 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.999964633, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| C1ORF186 PKN1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9992 | BioGRID | 2231683 | |
| C1ORF186 PKN1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9978 | BioGRID | 3118338 | |
| C1ORF186 PKN1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.6232 | BioGRID | 3286500 |
Curated By
- BioGRID