Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification and selected reaction monitoring (SRM) quantification of endocytosis factors associated with Numb.

Krieger JR, Taylor P, Gajadhar AS, Guha A, Moran MF, McGlade CJ

Numb is an endocytic adaptor protein that regulates the endocytosis and trafficking of transmembrane receptors including Notch, E-cadherin, and integrins. Vertebrate Numb is alternatively spliced at exons 3 and 9 to give rise to four protein isoforms. Expression of these isoforms varies at different developmental stages, and although the function of Numb isoforms containing exon 3 has been studied, the ... [more]

Mol. Cell Proteomics Feb. 01, 2013; 12(2);499-514 [Pubmed: 23211419]

Throughput

  • Low Throughput

Curated By

  • BioGRID