BAIT
GLE2
RAE1, RNA export factor GLE2, L000004084, YER107C
RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
GO Process (5)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
- nuclear pore distribution [IMP]
- poly(A)+ mRNA export from nucleus [IMP]
- posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery [IMP]
- regulation of mRNA export from nucleus in response to heat stress [IMP]
- transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [IMP]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
NPL3
MTR13, MTS1, NAB1, NOP3, mRNA-binding protein NPL3, L000001270, YDR432W
RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress
GO Process (6)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- mRNA export from nucleus [IGI]
- mRNA splicing, via spliceosome [IGI, IMP]
- negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled [IDA, IMP]
- negative regulation of translation [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA, IMP]
- translational termination [IGI, IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2.
Multiple export receptors passage bound pre-ribosomes through nuclear pore complexes (NPCs) by transiently interacting with the Phe-Gly (FG) meshwork of their transport channels. Here, we reveal how the non-FG interacting yeast mRNA export factor Gly-Leu-FG lethal 2 (Gle2) functions in the export of the large pre-ribosomal subunit (pre-60S). Structure-guided studies uncovered conserved platforms used by Gle2 to export pre-60S: an ... [more]
Nucleic Acids Res. Sep. 01, 2013; 41(17);8266-79 [Pubmed: 23907389]
Throughput
- Low Throughput
Ontology Terms
- inviable (APO:0000112)
Additional Notes
- Figure 1
Curated By
- BioGRID