CAM1
Gene Ontology Biological Process
Gene Ontology Molecular Function
AT5G28300
Gene Ontology Biological Process
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Plant-specific trihelix transcription factor AtGT2L interacts with calcium/calmodulin and responds to cold and salt stresses.
GT factors are a family of plant-specific transcription factors with conserved trihelix DNA-binding domains that bind GT elements. By screening a cDNA expression library with (35)S-labeled recombinant calmodulin (CaM), we identified AtGT2L, a classic member of GT-2 subfamily, as a Ca(2+)-dependent CaM-binding protein. AtGT2L specifically targets the nucleus and possesses both transcriptional activation and DNA-binding abilities, implicating its function as ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID