An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Tonoplast CBL-CIPK calcium signaling network regulates magnesium homeostasis in Arabidopsis.

Tang RJ, Zhao FG, Garcia VJ, Kleist TJ, Yang L, Zhang HX, Luan S

Although Mg(2+) is essential for a myriad of cellular processes, high levels of Mg(2+) in the environment, such as those found in serpentine soils, become toxic to plants. In this study, we identified two calcineurin B-like (CBL) proteins, CBL2 and CBL3, as key regulators for plant growth under high-Mg conditions. The Arabidopsis mutant lacking both CBL2 and CBL3 displayed severe ... [more]

Proc. Natl. Acad. Sci. U.S.A. Mar. 10, 2015; 112(10);3134-9 [Pubmed: 25646412]

Throughput

Low Throughput

Additional Notes

BiFC assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CIPK23 CBL3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Lee SC (2007)	Low	-	BioGRID	-
CBL3 CIPK23	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Tang RJ (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CBL3

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium ion binding [ISS]protein binding [IPI]

Gene Ontology Cellular Component

CIPK23

Gene Ontology Biological Process

Gene Ontology Molecular Function

kinase activity [ISS]protein binding [IPI]protein serine/threonine kinase activity [IDA]