An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis of the 26S proteasome reveals its direct interaction with transit peptides of plastid protein precursors for their degradation.

Sako K, Yanagawa Y, Kanai T, Sato T, Seki M, Fujiwara M, Fukao Y, Yamaguchi J

The 26S proteasome is an ATP-dependent proteinase complex that is responsible for regulated proteolysis of polyubiquitinated proteins in eukaryotic cells. Here, we report novel 26S proteasome interacting proteins in Arabidopsis as revealed by LC-MS/MS analysis. We performed a two-step screening process that involved affinity purification of the 26S proteasome using Arabidopsis plants expressing a FLAG-tagged RPT2a subunit and partial purification ... [more]

J. Proteome Res. Jul. 03, 2014; 13(7);3223-30 [Pubmed: 24846764]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPT2A PAG1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	McWhite CD (2020)	High	0.9549	BioGRID	2911315

Curated By

BioGRID

Interaction Summary

RPT2A

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IGI, ISS]

Gene Ontology Cellular Component

PAG1