BAIT
RPT2A
26S PROTEASOME AAA-ATPASE SUBUNIT RPT2A, F19B15.70, F19B15_70, regulatory particle AAA-ATPase 2A, AT4G29040
regulatory particle AAA-ATPase 2A
GO Process (21)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
- female gamete generation [IGI]
- lateral root formation [IMP]
- leaf morphogenesis [IMP]
- maintenance of root meristem identity [IMP]
- male gamete generation [IGI]
- meristem growth [IMP]
- meristem structural organization [IMP]
- phyllome development [IMP]
- proteasome assembly [IMP]
- proteasome core complex assembly [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- regulation of gene silencing [IMP]
- response to cadmium ion [IEP]
- response to heat [IMP]
- response to misfolded protein [IMP]
- root cap development [IMP]
- root development [IMP]
- root morphogenesis [IMP]
- seedling development [IMP]
- shoot system development [IMP]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Arabidopsis thaliana (Columbia)
PREY
FTSH1
F14I3.14, F14I3_14, FTSH protease 1, AT1G50250
ATP-dependent zinc metalloprotease FTSH 1
GO Process (2)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Arabidopsis thaliana (Columbia)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Proteomic analysis of the 26S proteasome reveals its direct interaction with transit peptides of plastid protein precursors for their degradation.
The 26S proteasome is an ATP-dependent proteinase complex that is responsible for regulated proteolysis of polyubiquitinated proteins in eukaryotic cells. Here, we report novel 26S proteasome interacting proteins in Arabidopsis as revealed by LC-MS/MS analysis. We performed a two-step screening process that involved affinity purification of the 26S proteasome using Arabidopsis plants expressing a FLAG-tagged RPT2a subunit and partial purification ... [more]
J. Proteome Res. Jul. 03, 2014; 13(7);3223-30 [Pubmed: 24846764]
Throughput
- Low Throughput
Curated By
- BioGRID