Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Proteomic analysis of the 26S proteasome reveals its direct interaction with transit peptides of plastid protein precursors for their degradation.

Sako K, Yanagawa Y, Kanai T, Sato T, Seki M, Fujiwara M, Fukao Y, Yamaguchi J

The 26S proteasome is an ATP-dependent proteinase complex that is responsible for regulated proteolysis of polyubiquitinated proteins in eukaryotic cells. Here, we report novel 26S proteasome interacting proteins in Arabidopsis as revealed by LC-MS/MS analysis. We performed a two-step screening process that involved affinity purification of the 26S proteasome using Arabidopsis plants expressing a FLAG-tagged RPT2a subunit and partial purification ... [more]

J. Proteome Res. Jul. 03, 2014; 13(7);3223-30 [Pubmed: 24846764]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

AT2G20140

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [ISS]

Gene Ontology Cellular Component