An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Proteomic analysis of the 26S proteasome reveals its direct interaction with transit peptides of plastid protein precursors for their degradation.

Sako K, Yanagawa Y, Kanai T, Sato T, Seki M, Fujiwara M, Fukao Y, Yamaguchi J

The 26S proteasome is an ATP-dependent proteinase complex that is responsible for regulated proteolysis of polyubiquitinated proteins in eukaryotic cells. Here, we report novel 26S proteasome interacting proteins in Arabidopsis as revealed by LC-MS/MS analysis. We performed a two-step screening process that involved affinity purification of the 26S proteasome using Arabidopsis plants expressing a FLAG-tagged RPT2a subunit and partial purification ... [more]

J. Proteome Res. Jul. 03, 2014; 13(7);3223-30 [Pubmed: 24846764]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LTA2 CHIP	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Sako K (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CHIP

Gene Ontology Biological Process

Gene Ontology Molecular Function

chaperone binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

LTA2