An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

5-HT(6) receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia.

Meffre J, Chaumont-Dubel S, Mannoury la Cour C, Loiseau F, Watson DJ, Dekeyne A, Seveno M, Rivet JM, Gaven F, Deleris P, Herve D, Fone KC, Bockaert J, Millan MJ, Marin P

Cognitive deficits in schizophrenia severely compromise quality of life and are poorly controlled by current antipsychotics. While 5-HT(6) receptor blockade holds special promise, molecular substrates underlying their control of cognition remain unclear. Using a proteomic strategy, we show that 5-HT(6) receptors physically interact with several proteins of the mammalian target of rapamycin (mTOR) pathway, including mTOR. Further, 5-HT(6) receptor activation ... [more]

EMBO Mol Med Oct. 01, 2012; 4(10);1043-56 [Pubmed: 23027611]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HTR6 ATXN10	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Sokolina K (2017)	High	-	BioGRID	2827532

Curated By

BioGRID

Interaction Summary

HTR6

Gene Ontology Biological Process

Gene Ontology Molecular Function

histamine receptor activity [TAS]protein binding [IPI]

Gene Ontology Cellular Component

ATXN10