BAIT

CLU

APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, AAG4
clusterin
GO Process (35)
GO Function (4)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens
PREY

SEMG1

CT103, SEMG, SGI, dJ172H20.2
semenogelin I
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Characterization of an eppin protein complex from human semen and spermatozoa.

Wang Z, Widgren EE, Richardson RT, O'Rand MG

Eppin (SPINLW1; serine peptidase inhibitor-like with Kunitz and WAP domains 1 (eppin); epididymal protease inhibitor) coats the surface of human ejaculate spermatozoa and originates from Sertoli and epididymal epithelial cells. In this study, we have isolated native eppin from ejaculate supernatants (seminal plasma) and washed ejaculate spermatozoa using column chromatography and two-dimensional SDS-PAGE, and identified by mass spectrometry and Western ... [more]

Biol. Reprod. Sep. 01, 2007; 77(3);476-84 [Pubmed: 17567961]

Throughput

  • Low Throughput

Curated By

  • BioGRID