BAIT

BNI1

PPF3, SHE5, formin BNI1, L000000190, YNL271C
Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
Saccharomyces cerevisiae (S288c)
PREY

PIN4

MDT1, YBL051C
Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Cell-cycle regulation of formin-mediated actin cable assembly.

Miao Y, Wong CC, Mennella V, Michelot A, Agard DA, Holt LJ, Yates JR, Drubin DG

Assembly of appropriately oriented actin cables nucleated by formin proteins is necessary for many biological processes in diverse eukaryotes. However, compared with knowledge of how nucleation of dendritic actin filament arrays by the actin-related protein-2/3 complex is regulated, the in vivo regulatory mechanisms for actin cable formation are less clear. To gain insights into mechanisms for regulating actin cable assembly, ... [more]

Proc. Natl. Acad. Sci. U.S.A. Nov. 19, 2013; 110(47);E4446-55 [Pubmed: 24133141]

Throughput

  • High Throughput

Additional Notes

  • Figure 3
  • proteins identified using MS from formin-reconstituted actin cables

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BNI1 PIN4
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

High-BioGRID
108928
BNI1 PIN4
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

High-BioGRID
450027

Curated By

  • BioGRID