BAIT
BNI1
PPF3, SHE5, formin BNI1, L000000190, YNL271C
Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
GO Process (9)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
- actin filament bundle assembly [IGI, IMP]
- actin nucleation [IDA]
- actomyosin contractile ring actin filament bundle assembly [IMP]
- barbed-end actin filament capping [IDA]
- budding cell apical bud growth [IGI, IMP]
- establishment of mitotic spindle orientation [IMP]
- formin-nucleated actin cable assembly [IDA, IGI, IMP]
- positive regulation of actin cytoskeleton reorganization [IGI, IMP]
- regulation of protein localization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CAP2
F-actin-capping protein subunit beta, L000000215, YIL034C
Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
GO Process (2)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function- actin filament binding [IGI, IMP, IPI, ISS]
- actin filament binding [IGI, IMP, IPI, ISS]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Cell-cycle regulation of formin-mediated actin cable assembly.
Assembly of appropriately oriented actin cables nucleated by formin proteins is necessary for many biological processes in diverse eukaryotes. However, compared with knowledge of how nucleation of dendritic actin filament arrays by the actin-related protein-2/3 complex is regulated, the in vivo regulatory mechanisms for actin cable formation are less clear. To gain insights into mechanisms for regulating actin cable assembly, ... [more]
Proc. Natl. Acad. Sci. U.S.A. Nov. 19, 2013; 110(47);E4446-55 [Pubmed: 24133141]
Throughput
- High Throughput
Additional Notes
- Figure 3
- proteins identified using MS from formin-reconstituted actin cables
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BNI1 CAP2 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 452441 |
Curated By
- BioGRID