BAIT

GW

CG11484, CG31992, CG9905, Dmel\CG31992, GW182, anon-EST:Posey205, dGW182, Dmel_CG31992
gawky
Drosophila melanogaster
PREY

CG11486

Dmel\CG11486, PAN3, Dmel_CG11486
CG11486 gene product from transcript CG11486-RM
GO Process (1)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Drosophila melanogaster

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets.

Braun JE, Huntzinger E, Fauser M, Izaurralde E

miRNAs are posttranscriptional regulators of gene expression that associate with Argonaute and GW182 proteins to repress translation and/or promote mRNA degradation. miRNA-mediated mRNA degradation is initiated by deadenylation, although it is not known whether deadenylases are recruited to the mRNA target directly or by default, as a consequence of a translational block. To answer this question, we performed a screen ... [more]

Mol. Cell Oct. 07, 2011; 44(1);120-33 [Pubmed: 21981923]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GW CG11486
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID