BAIT

PRP4

RNA4, L000001497, YPR178W
Splicing factor; component of the U4/U6-U5 snRNP complex
UPS Project
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SNR7-L

U5L, SNR7
U5 spliceosomal RNA (U5 snRNA), long form; associated with spliceosome
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasis.

Huang YH, Chung CS, Kao DI, Kao TC, Cheng SC

The yeast Sad1 protein was previously identified in a screen for factors involved in the assembly of the U4/U6 di-snRNP particle. Sad1 is required for pre-mRNA splicing both in vivo and in vitro, and its human orthologue has been shown to associate with U4/U6.U5 tri-snRNP. We show here that Sad1 plays a role in maintaining a functional form of the ... [more]

Mol. Cell. Biol. Jan. 01, 2014; 34(2);210-20 [Pubmed: 24190974]

Throughput

  • Low Throughput

Additional Notes

  • Figure 1A

Curated By

  • BioGRID