An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Trans-Golgi network-located AP1 gamma adaptins mediate dileucine motif-directed vacuolar targeting in Arabidopsis.

Wang X, Cai Y, Wang H, Zeng Y, Zhuang X, Li B, Jiang L

Membrane proteins on the tonoplast are indispensible for vacuolar functions in plants. However, how these proteins are transported to the vacuole and how they become separated from plasma membrane proteins remain largely unknown. In this study, we used Arabidopsis thaliana vacuolar ion transporter1 (VIT1) as a reporter to study the mechanisms of tonoplast targeting. We showed that VIT1 reached the ... [more]

Plant Cell Oct. 01, 2014; 26(10);4102-18 [Pubmed: 25351491]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VIT1 AP1	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Wang X (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

VIT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

iron ion transmembrane transporter activity [IGI]

Gene Ontology Cellular Component

AP1