BAIT

HSP21

T29A15.160, T29A15_160, heat shock protein 21, AT4G27670
heat shock protein 21
GO Process (3)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

MDH

MALATE DEHYDROGENASE, AT3G47520
malate dehydrogenase
Arabidopsis thaliana (Columbia)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Probing the transient interaction between the small heat-shock protein Hsp21 and a model substrate protein using crosslinking mass spectrometry.

Lambert W, Rutsdottir G, Hussein R, Bernfur K, Kjellstroem S, Emanuelsson C

Small heat-shock protein chaperones are important players in the protein quality control system of the cell, because they can immediately respond to partially unfolded proteins, thereby protecting the cell from harmful aggregates. The small heat-shock proteins can form large polydisperse oligomers that are exceptionally dynamic, which is implicated in their function of protecting substrate proteins from aggregation. Yet the mechanism ... [more]

Cell Stress Chaperones Jan. 01, 2013; 18(1);75-85 [Pubmed: 22851138]

Throughput

  • Low Throughput

Curated By

  • BioGRID