BAIT

GF14 PHI

14-3-3 PROTEIN G-BOX FACTOR14 PHI, 14-3-3PHI, GENERAL REGULATORY FACTOR 4, GF14 protein phi chain, GRF4, T32G9.30, T32G9_30, AT1G35160
14-3-3-like protein GF14 phi
GO Process (1)
GO Function (2)
GO Component (6)
Arabidopsis thaliana (Columbia)
PREY

CINV1

F15O4.33, cytosolic invertase 1, AT1G35580
alkaline/neutral invertase CINV1
Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Light modulated activity of root alkaline/neutral invertase involves the interaction with 14-3-3 proteins.

Gao J, van Kleeff PJ, Oecking C, Li KW, Erban A, Kopka J, Hincha DK, de Boer AH

Alkaline/neutral invertases (A/N-Invs) are now recognized as essential proteins in plant life. They catalyze the irreversible breakdown of sucrose into glucose and fructose and thus supply the cells with energy as well as signaling molecules. In this study we report on a mechanism that affects the activity of the cytosolic invertase AtCINV1 (At-A/N-InvG or AT1G35580). We demonstrate that Ser547 at ... [more]

Plant J. Dec. 01, 2014; 80(5);785-96 [Pubmed: 25256212]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GF14 PHI CINV1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-
CINV1 GF14 PHI
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID