An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The homeodomain-leucine zipper ATHB23, a phytochrome B-interacting protein, is important for phytochrome B-mediated red light signaling.

Choi H, Jeong S, Kim DS, Na HJ, Ryu JS, Lee SS, Nam HG, Lim PO, Woo HR

Phytochromes are red (R)/far-red (FR) photoreceptors that are central to the regulation of plant growth and development. Although it is well known that photoactivated phytochromes are translocated into the nucleus where they interact with a variety of nuclear proteins and ultimately regulate genome-wide transcription, the mechanisms by which these photoreceptors function are not completely understood. In an effort to enhance ... [more]

Physiol Plant Aug. 21, 2013; 0(0); [Pubmed: 23964902]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PHYB PIL1	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Choi H (2013)	Low	-	BioGRID	1239270

Curated By

BioGRID

Interaction Summary

PHYB

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled photoreceptor activity [ISS]far-red light photoreceptor activity [IDA]identical protein binding [IPI]protein binding [IPI]protein histidine kinase activity [ISS]red light photoreceptor activity [IDA]red or far-red light photoreceptor activity [IMP]signal transducer activity [ISS]

Gene Ontology Cellular Component

PIL1