An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A mechanism for retromer endosomal coat complex assembly with cargo.

Harrison MS, Hung CS, Liu TT, Christiano R, Walther TC, Burd CG

Retromer is an evolutionarily conserved protein complex composed of the VPS26, VPS29, and VPS35 proteins that selects and packages cargo proteins into transport carriers that export cargo from the endosome. The mechanisms by which retromer is recruited to the endosome and captures cargo are unknown. We show that membrane recruitment of retromer is mediated by bivalent recognition of an effector ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 07, 2014; 111(1);267-72 [Pubmed: 24344282]

Throughput

Low Throughput

Additional Notes

Figure S3

Curated By

BioGRID

Interaction Summary

VPS35

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein transporter activity [IBA]

Gene Ontology Cellular Component

SLC11A2