Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

cGMP-dependent protein kinase IÎ² interacts with p44/WDR77 to regulate androgen receptor-driven gene expression.

Zhou L, Hosohata K, Gao S, Gu Z, Wang Z

The androgen receptor (AR) pathway plays critical roles in controlling differentiation and proliferation of prostate epithelial cells. We previously identified a novel AR cofactor, p44/WDR77, which specifically enhances AR transcriptional activity in the prostate gland and prostate cancer. To further elucidate p44/WDR77's role in the AR signaling pathway, we conducted a yeast two-hybrid screening and identified cGMP-dependent protein kinase (PKG) ... [more]

PLoS ONE Jun. 12, 2013; 8(6);e63119 [Pubmed: 23755100]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
WDR77 PRKG2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zhou L (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

WDR77

Gene Ontology Biological Process

Gene Ontology Molecular Function

ligand-dependent nuclear receptor transcription coactivator activity [IGI]protein binding [IPI]

Gene Ontology Cellular Component

PRKG2