An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

cGMP-dependent protein kinase IÎ² interacts with p44/WDR77 to regulate androgen receptor-driven gene expression.

Zhou L, Hosohata K, Gao S, Gu Z, Wang Z

The androgen receptor (AR) pathway plays critical roles in controlling differentiation and proliferation of prostate epithelial cells. We previously identified a novel AR cofactor, p44/WDR77, which specifically enhances AR transcriptional activity in the prostate gland and prostate cancer. To further elucidate p44/WDR77's role in the AR signaling pathway, we conducted a yeast two-hybrid screening and identified cGMP-dependent protein kinase (PKG) ... [more]

PLoS ONE Jun. 12, 2013; 8(6);e63119 [Pubmed: 23755100]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
WDR77 PRKG2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Zhou L (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

WDR77

Gene Ontology Biological Process

Gene Ontology Molecular Function

ligand-dependent nuclear receptor transcription coactivator activity [IGI]protein binding [IPI]

Gene Ontology Cellular Component

PRKG2