BAIT

CIK1

L000000335, YMR198W
Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

DAM1

L000004420, YGR113W
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The signaling network that silences the spindle assembly checkpoint upon the establishment of chromosome bipolar attachment.

Jin F, Wang Y

Improper kinetochore attachments activate the spindle assembly checkpoint (SAC) to prevent anaphase onset, but it is poorly understood how this checkpoint is silenced to allow anaphase onset. Chromosome bipolar attachment applies tension on sister kinetochores, and the lack of tension delays anaphase onset. In budding yeast, the delay induced by tension defects depends on the intact SAC as well as ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 24, 2013; 110(52);21036-41 [Pubmed: 24324173]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cell size (APO:0000052)
  • phenotype: protein/peptide accumulation (APO:0000149)

Additional Notes

  • Figure 2
  • MAD1 deletion suppresses delayed Pds1 degradation in dam1-3D mutant
  • dam1-3A mutation suppresses delayed Pds1 degradation caused by Cik1 coiled-coil domain overexpression
  • dam1-3A mutation suppresses increase in large budded cells caused by Cik1 coiled-coil domain overexpression

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DAM1 CIK1
Dosage Growth Defect
Dosage Growth Defect

A genetic interaction is inferred when over expression or increased dosage of one gene causes a growth defect in a strain that is mutated or deleted for another gene.

Low-BioGRID
1240090
DAM1 CIK1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.187BioGRID
382534
DAM1 CIK1
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
1240087

Curated By

  • BioGRID