BAIT

CPR2

CYP2, peptidylprolyl isomerase CPR2, L000000463, YHR057C
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR2 has a paralog, CPR5, that arose from the whole genome duplication; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Saccharomyces cerevisiae (S288c)
PREY

SERPINA1

A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, PRO0684
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Homo sapiens

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Retarded protein folding of the human Z-type α₁-antitrypsin variant is suppressed by Cpr2p.

Jung CH, Kim YH, Lee K, Im H

The human Z-type α1-antitrypsin variant has a strong tendency to accumulate folding intermediates due to extremely slow protein folding within the endoplasmic reticulum (ER) of hepatocytes. Human α1-antitrypsin has 17 peptidyl-prolyl bonds per molecule; thus, the effect of peptidyl-prolyl isomerases on Z-type α1-antitrypsin protein folding was analyzed in this study. The protein level of Cpr2p, a yeast ER peptidyl-prolyl isomerase, ... [more]

Biochem. Biophys. Res. Commun. Feb. 28, 2014; 445(1);191-5 [Pubmed: 24502947]

Throughput

  • Low Throughput

Additional Notes

  • Figure 1
  • Overexpression of Z-type alpha1-AT causes a growth defect in cpr2 mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CPR2 SERPINA1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1240336

Curated By

  • BioGRID