BAIT
MMT2
MFT2, L000004108, YPL224C
Putative metal transporter involved in mitochondrial iron accumulation; MMT2 has a paralog, MMT1, that arose from the whole genome duplication
GO Process (1)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
AFT1
RCS1, DNA-binding transcription factor AFT1, L000002660, L000001594, YGL071W
Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
GO Process (6)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- chromosome segregation [IMP]
- establishment of mitotic sister chromatid cohesion [IMP]
- meiotic chromosome segregation [IMP]
- positive regulation of iron ion transport [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation [IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Rescue
A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.
Publication
Expression of the yeast cation diffusion facilitators Mmt1 and Mmt2 affects mitochondrial and cellular iron homeostasis: evidence for mitochondrial iron export.
Mmt1 and Mmt2 are highly homologous yeast members of the cation diffusion facilitator transporter family localized to mitochondria. Overexpression of MMT1/2 led to changes in cellular metal homeostasis (increased iron sensitivity, decreased cobalt sensitivity, increased sensitivity to copper), oxidant generation, and increased sensitivity to H2O2. The phenotypes due to overexpression of MMT1&2 were similar to that seen in cells with ... [more]
J. Biol. Chem. Jun. 13, 2014; 289(24);17132-41 [Pubmed: 24798331]
Throughput
- Low Throughput
Ontology Terms
- phenotype: metal resistance (APO:0000090)
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- AFT1 deletion suppresses cobalt-sensitivity in cells overexpressing MMT1 and MMT2
- Figure 3
- genetic complex
Curated By
- BioGRID