BAIT

PTPN5

PTPSTEP, STEP
protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens
PREY

MAPK1

9030612K14Rik, AA407128, AU018647, C78273, ERK, Erk2, MAPK2, PRKM2, Prkm1, p41mapk, p42mapk
mitogen-activated protein kinase 1
GO Process (35)
GO Function (12)
GO Component (18)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

STEP61 is a substrate of the E3 ligase parkin and is upregulated in Parkinson's disease.

Kurup PK, Xu J, Videira RA, Ononenyi C, Baltazar G, Lombroso PJ, Nairn AC

Parkinson's disease (PD) is characterized by the degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc). The loss of SNc dopaminergic neurons affects the plasticity of striatal neurons and leads to significant motor and cognitive disabilities during the progression of the disease. PARK2 encodes for the E3 ubiquitin ligase parkin and is implicated in genetic and sporadic PD. ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 27, 2015; 112(4);1202-7 [Pubmed: 25583483]

Throughput

  • Low Throughput

Curated By

  • BioGRID