RPO21
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
INO80
Gene Ontology Biological Process
- DNA repair [IDA, IMP]
- chromatin remodeling [IDA, IMP]
- chromatin silencing at telomere [IMP]
- histone exchange [IDA]
- nucleosome mobilization [IMP]
- nucleosome positioning [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of chromosome segregation [IMP]
- regulation of transcription from RNA polymerase II promoter in response to stress [IMP]
- telomere maintenance via recombination [IGI]
- transcription from RNA polymerase II promoter [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Threonine-4 of the budding yeast RNAP II CTD couples transcription with Htz1-mediated chromatin remodeling.
The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAP II) consists of repeated YSPTSPS heptapeptides and connects transcription with cotranscriptional events. Threonine-4 (Thr4) of the CTD repeats has been shown to function in histone mRNA 3'-end processing in chicken cells and in transcriptional elongation in human cells. Here, we demonstrate that, in budding yeast, Thr4, although ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- Figure 5
- rpo21-T4V
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| INO80 RPO21 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1510479 | |
| RPO21 INO80 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1510480 | |
| INO80 RPO21 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2054 | BioGRID | 1933822 |
Curated By
- BioGRID